Employment

February, 2011 -  Present
Adjunct Assistant Professor

2003 -  2010
Cellular and Molecular Biology Laboratory instructor

Co-instructor of an advanced biology course Recombinant DNA Technology ; Biology Department, Brooklyn College

Co-creator involved in the development of a master syllabus for and co-instructor of an innovative advanced biology laboratory course with Dr.Dan Eshel ; Biology Department, Brooklyn College
Field botany lab instructor ; Biology Department, Brooklyn College

2000 -  2003

Summer sessions Instructor of preparatory classes in biology, inorganic and organic chemistry as a part of the Summer Enrichment Program, designed to prepare incoming BA - M.D. students for science courses ; Dean of Undergraduate Studies, Brooklyn College
Biology tutor ; Learning Center, Brooklyn College
Summer and winter sessions Math and English tutor for the CUNY Entrance Exam ; Dean of Undergraduate Studies, Brooklyn College
High school
Dental Assistant ; Dr.Jeffrey M. Galler, MAGD

Awards, Honors and Fellowships
Graduate years Master and Doctoral programs

Recipient of the 2010 Brooklyn College Kappa Phi Club Award for outstanding achievement

Recipient of the CUNY Graduate Center Research Grant Award for Graduate Students for the maximum amount 2007

2003 -  2010

2003 -  2010

2003 -  2010

Student Travel Stipend for conferences attended 2006 - 2010

Received a stipend for participating in a Research Workshop given by the English Department for fellows teaching research writing skills to undergraduate students 2008

Undergraduate years

2000 -  2003

1999 -  2003

Shohat-Tal, A. and Eshel, D. Cell Cycle Regulators Interact with Pathways that Modulate Microtubule Stability in Yeast. Eukaryotic Cell. In press.

Abstract
The integrity of mitosis is dependent upon strict regulation of microtubule stability and dynamics. though much information is accumulated on regulators of the microtubule cytoskeleton, our knowledge of the specific pathways involved is limited. Here we designed genetic screens to identify regulators of microtubule stability that are dispensable in the wild-type become essential under microtubule disrupting conditions. We found that the transcriptional cofactor Swi6p and activator Swi4p and the G2/M-specific cyclin Clb2p, are required in a microtubule-destabilizing environment. Swi6p and Swi4p can combine as a transcriptional complex, called the SBF complex that is functionally homologous to the metazoan DP1/2 - E2F complex and which controls the G1/S transition through the genes it regulates. We show that Swi6p s contribution to microtubule stability is either dependent or independent of the SBF complex. The SBF-dependent pathway requires downregulation of SBF complex levels and may re-route the transcriptional program in favor of greater microtubule stability. This pathway is triggered by overexpression of Fcp1p, a phosphatase in the general transcription machinery or by expression of an allele of SWI6 that is associated with reduced transcription from SBF-controlled promoters. The SBF-independent pathway is activated by a nuclear allele of Swi6p. Our results introduce novel roles in microtubule stability for genes whose participation in the process may be masked under normal conditions, acquire a dominant role when microtubule stability is compromised.

Oral and Poster Presentation
1 Shohat, A. and Eshel, D. Regulation of Microtubule stability in yeast. Cold Spring Harbor Laboratory Yeast Cell Biology meeting, 2009. Poster presentation
2 Shohat, A. and Eshel, D. Regulation of Microtubule Stability and Dynamics in Saccharomyces cereviaisae. CUNY Graduate Center MCD seminars, October 2008. Oral presentation
3 Shohat, A. and Eshel, D. Genes involved in the regulation of microtubule stability and dynamics in Saccharomyces cerevisiae. Gordon Research Conference on Plant and Fungal Cytoskeleton, 2008, Lucca, Italy. Poster presentation
4 Shohat, A. and Eshel, D. Identification of genes involved in the regulation of microtubule stability in yeast. Brooklyn College annual SCORE Support of Continuous Research Excellence Retreat at the New York Academy of Sciences, 2008. Oral presentation
5 Shohat, A. and Eshel, D. Genes involved in the regulation of microtubule stability and dynamics in Saccharomyces cerevisiae. Eighth Annual Celebration of Science, Engineering and Mathematics at the CUNY Graduate Center, 2007. Poster presentation
6 Shohat, A. and Eshel, D.A genetic screen for genes involved in the regulation of microtubule stability and dynamics in Saccharomyces cerevisiae. Brooklyn College annual SCORE Retreat at the New York Academy of Sciences, 2007. Poster presentation

BD FACSCanto Operator certified technician

Languages
English, Hebrew

Genetics : Gene knockout/ replacement
Gene tagging ie, with fluorescent markers
Insertional mutagenesis ie, transposon-mediated
Design of genetic screens
Drug sensitivity assays
Yeast genetics crossing opposite mating-types, sporulation, tetrad dissection by micromanipulation
Cell cycle studies : Cell culture synchronizations ? - factor, hydroxyurea, nocodazole-mediated
Flow cytometry and propidium iodide staining
Fluorescence Microscopy : Fluorescent gene tagging
DAPI staining of fluorescently-tagged samples
Plasmid manipulation : Subcloning and vector construction
Site-directed mutagenesis
Agarose gel electrophoresis and transformation
PCR : PCR-mediated gene disruption/tagging by homologous recombination
Thermal Asymmetric Interlaced TAIL PCR
Protein studies : Protein extraction
Spectroscopy
SDS-PAGE
Western blots
ELISA
DNA studies : Plasmid and genomic preps
Southern blots

May 5, 2011 : Invited to give a talk entitled Science and Biotechnology as a career choice at the Yeshivah of Flatbush Joel Braverman High School Career Fair

September 2009 - December 2010 : Founder and Chair of the Jewish Connection Group at the CUNY Graduate Center, a chartered organization aimed at stimulating dialogue and philosophical discussions of Jewish tradition and culture

2008 -  2010
the Advisory Committee for the Biology Executive

2008 -  2011

2008 -  2009

2007 -  2008
At Large Representative

2007 - 2010 : Interviewed prospective freshmen candidates for the Downstate Medical Center-affiliated BA - M.D. program The BA - M.D. Program is a combined undergraduate and medical degree program run jointly by Brooklyn College and SUNY Downstate Medical Center. Dr.Ken Miyano, Former Director of the BA - MD. Program
May 21, 2007 : Invited to give a talk entitled Scientific Research : How is it done ? at the Yeshivah of Flatbush Joel Braverman High School Science Symposium

November 14, 2006 : Gave a talk entitled Yeast microtubules and the cell cycle at the Brooklyn College Chemistry Society and the NBSSO National Black Science Students Organization

2003 -  Present

2000 -  2003

Education